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UHN Nuclear Magnetic Resonance Core Facility

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Welcome to UHN Nuclear Magnetic Resonance Core Facility

The UHN BioNMR Core Facility is an open access research facility located in the Princess Margaret Cancer Research Tower in Toronto. The NMR Core Facility is equipped with Cutting-edge NMR spectrometers allowing researchers to

  • Characterize bio-molecular structures at the atomic resolution with exquisite details
  • Identify protein interactions with binding partners in order to understand their architecture
  • Study protein dynamic
  • Study protein post-translational modifications acting as molecular switches
  • Study structure-function relationships of biological proteins to gain insight into biological processes in relation to human disease
  • Screening of High-throughput drug-like leads for novel therapeutic targets
  • Study Metabolites of interest

The Core facility houses high-field NMR spectrometers equipped with cryogenically cooled probes (1H or 13C optimized) for enhanced sensitivity.

The core facility is overseen by Dr. Geneviève Seabrook- Staff Scientist and NMR Core Facility Manager.

photo of Geneviève Seabrook

Dr. Geneviève Seabrook
Staff Scientist- NMR Core Facility Manager

Our Services & Equipment

Services

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Services

  • Free project consultation
  • Help with experimental design
  • Full support service
  • NMR Training
  • Our services include:
    • Collecting data
    • Data analysis
    • Data Interpretation

Access to internal and external users
The Core Facility runs 24/7

NMR Spectrometers

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NMR Spectrometers

Three Bruker NMR spectrometers:

  • Bruker NEO 800MHz
  • Bruker Avance HD 700MHz
  • Bruker Avance III 600MHz

Our NMR Core facility is equipped with three NMR spectrometers. For more information regarding the specificity of each NMR spectrometer, please see section NMR spectrometers.

Bruker 800 MHz NMR Spectrometer

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Bruker 800 MHz NMR Spectrometer

  • Magnet: Bruker 800 MHz US2 (Ultra Stabilized, Ultra Shielded)
  • Console: NEO
  • Probes:
    • 5 mm TXO CryoProbe Z-Gradient;
      13C optimized
    • 5 mm TCI CryoProbes Z-Gradient
    • 5 mm TXI room temperature probe, Z-Gradient
  • Four channels (1H, 15N, 13C, 2H)
  • Optimized for protein structure, protein-protein interaction, protein dynamic, protein-membrane interaction, unstructured regions/proteins (IDPs).

Bruker 700 MHz NMR Spectrometer

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Bruker 700 MHz NMR Spectrometer

  • Magnet: Bruker 700 MHz Ascend
  • Console: Avance III HD
  • Probes: 5 mm TCI CryoProbe, XYZ-Gradients
  • Four channels (1H, 15N, 13C, 2H)
  • Sample changer:
    • Cooled Sample Case: 24 samples (5mm/3mm tubes)
    • Samples are kept at 4 in the sample case
    • Controlled through IconNMR
  • Optimized for routine protein studies

Bruker 600 MHz NMR Spectrometer

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Bruker 600 MHz NMR Spectrometer

  • Magnet: Bruker 600 MHz US Plus
  • Console: Avance III
  • Probes:
    • 1.7 mm TCI Micro-CryoProbe, Z-gradient
    • 5 mm TCI CryoProbe, Z-gradient
    • 5 mm TXI room temperature probe, Z-gradient
  • Four channels (1H, 15N, 13C, 2H)
  • Auto Sampler: Sample Jet
    • Capacity: 5 racks of 96 samples (1 mm, 1.7 mm, 3 mm, 5 mm diameter) + 30 samples
    • Samples are kept at 4 in the Sample Jet carousel
    • Controlled through IconNMR
  • Optimized for NMR screening applications such as small-molecule screening, fragment-based screening, DNA-encoded libraries

Gilson Sample Handler

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Gilson Sample Handler

  • Used for NMR High-Throughput Screening
    • 1.7 mm NMR tubes; vol = 35 µL
    • 3 mm NMR tubes; vol = 185 µL
    • 5 mm NMR tubes; vol = 500 µL

Our Services & Equipment

Services

+

Services

  • Free project consultation
  • Help with experimental design
  • Full support service
  • NMR Training
  • Our services include:
    • Collecting data
    • Data analysis
    • Data Interpretation

Access to internal and external users
The Core Facility runs 24/7

NMR Spectrometers

+

NMR Spectrometers

Three Bruker NMR spectrometers:

  • Bruker NEO 800MHz
  • Bruker Avance HD 700MHz
  • Bruker Avance III 600MHz

Our NMR Core facility is equipped with three NMR spectrometers. For more information regarding the specificity of each NMR spectrometer, please see section NMR spectrometers below.

Bruker 800 MHz NMR Spectrometer

+

Bruker 800 MHz NMR Spectrometer

  • Magnet: Bruker 800 MHz US2 (Ultra Stabilized, Ultra Shielded)
  • Console: NEO
  • Probes:
    • 5 mm TXO CryoProbe Z-Gradient;
      13C optimized (4171:1 ratio)
    • 5 mm TCI CryoProbes Z-Gradient
    • 5 mm TXI room temperature probe, Z-Gradient
  • Four channels (1H, 15N, 13C, 2H)
  • Optimized for protein structure, protein-protein interaction, protein dynamic, protein-membrane interaction, unstructured regions/proteins (IDPs).

Bruker 700 MHz NMR Spectrometer

+

Bruker 700 MHz NMR Spectrometer

  • Magnet: Bruker 700 MHz Ascend
  • Console: Avance III HD
  • Probes: 5 mm TCI CryoProbe, XYZ-Gradients
  • Four channels (1H, 15N, 13C, 2H)
  • Sample changer:
    • Cooled Sample Case: 24 samples (5mm/3mm tubes)
    • Samples are kept at 4 in the sample case
    • Controlled through IconNMR
  • Optimized for routine protein studies

Bruker 600 MHz NMR Spectrometer

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Bruker 600 MHz NMR Spectrometer

  • Magnet: Bruker 600 MHz US Plus
  • Console: Avance III
  • Probes:
    • 1.7 mm TCI Micro-CryoProbe, Z-gradient
    • 5 mm TCI CryoProbe, Z-gradient
    • 5 mm TXI room temperature probe, Z-gradient
  • Four channels (1H, 15N, 13C, 2H)
  • Auto Sampler: Sample Jet
    • Capacity: 5 racks of 96 samples (1 mm, 1.7 mm, 3 mm, 5 mm diameter) + 30 samples
    • Samples are kept at 4 in the Sample Jet carousel
    • Controlled through IconNMR
  • Optimized for NMR screening applications such as small-molecule screening, fragment-based screening, DNA-encoded libraries

Gilson Sample Handler

+

Gilson Sample Handler

  • Used for NMR High-Throughput Screening
    • 1.7 mm NMR tubes; vol = 35 µL
    • 3 mm NMR tubes; vol = 185 µL
    • 5 mm NMR tubes; vol = 500 µL

Services

  • We offer free project consultation to discuss project feasibility (including sample preparation and NMR experiments) and offer expert advice.
  • We offer a full service for researchers who may not be able to perform their own experiments.
  • We train graduates, PhD students and post-doctoral fellows to run their own NMR experiments and we provide support for the data analysis when needed.
  • We collect, analyze data and send a full report when requested by the researcher.
  • The NMR spectrometers are available to internal and external researchers. The use of the NMR instruments independently is contingent upon training and approval of each individual by the NMR Core Facility Manager Dr. G. Seabrook.

FAQ

It will depend on the project. We will discuss the choice of the spectrometer once we understand the goal of the project.

We can screen a few thousands (~3000) of compounds in just a few days.

Please contact Dr. G. Seabrook.

Dr. G. Seabrook will provide the information at the time.

We have an on-line calendar. The access will be provided after confirmation of the spectrometers access authorization.

Dr. Geneviève Seabrook will provide training.

Contact Us

Nuclear Magnetic Resonance Core Facility

Nuclear Magnetic Resonance Core Facility is located at:

MaRS, Princess Margaret Cancer Research Tower
East Tower, 101 College Street
Toronto, Ontario, Canada
M5G 1L7

Image credit: UHN's Research Strategy team