Welcome to UHN Nuclear Magnetic Resonance Core Facility
The UHN BioNMR Core Facility is an open access research facility
located in the Princess Margaret Cancer Research Tower in Toronto.
The NMR Core Facility is equipped with Cutting-edge NMR
spectrometers allowing researchers to
Characterize bio-molecular structures at the atomic resolution
with exquisite details
Identify protein interactions with binding partners in order to
understand their architecture
Study protein dynamic
Study protein post-translational modifications acting as
molecular switches
Study structure-function relationships of biological proteins to
gain insight into biological processes in relation to human
disease
Screening of High-throughput drug-like leads for novel
therapeutic targets
Study Metabolites of interest
The Core facility houses high-field NMR spectrometers equipped
with cryogenically cooled probes (1H or 13C
optimized) for enhanced sensitivity.
The core facility is overseen by Dr. Geneviève Seabrook- Staff
Scientist and NMR Core Facility Manager.
Dr. Geneviève Seabrook Staff Scientist- NMR Core Facility Manager
Access to internal and external users
The Core Facility runs 24/7
NMR Spectrometers
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NMR Spectrometers
Three Bruker NMR spectrometers:
Bruker NEO 800MHz
Bruker Avance HD 700MHz
Bruker Avance III 600MHz
Our NMR Core facility is equipped with three NMR
spectrometers. For more information regarding the
specificity of each NMR spectrometer, please see section
NMR spectrometers.
Bruker 800 MHz NMR Spectrometer
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Bruker 800 MHz NMR Spectrometer
Magnet: Bruker 800 MHz US2 (Ultra Stabilized, Ultra
Shielded)
Console: NEO
Probes:
5 mm TXO CryoProbe Z-Gradient; 13C optimized
5 mm TCI CryoProbes Z-Gradient
5 mm TXI room temperature probe, Z-Gradient
Four channels (1H, 15N,
13C, 2H)
Optimized for protein structure, protein-protein
interaction, protein dynamic, protein-membrane
interaction, unstructured regions/proteins (IDPs).
Bruker 700 MHz NMR Spectrometer
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Bruker 700 MHz NMR Spectrometer
Magnet: Bruker 700 MHz Ascend
Console: Avance III HD
Probes: 5 mm TCI CryoProbe, XYZ-Gradients
Four channels (1H, 15N,
13C, 2H)
Sample changer:
Cooled Sample Case: 24 samples (5mm/3mm tubes)
Samples are kept at 4℃ in the
sample case
Controlled through IconNMR
Optimized for routine protein studies
Bruker 600 MHz NMR Spectrometer
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Bruker 600 MHz NMR Spectrometer
Magnet: Bruker 600 MHz US Plus
Console: NEO
Probes:
1.7 mm TCI Micro-CryoProbe, Z-gradient
5 mm TCI CryoProbe, Z-gradient
5 mm TXI room temperature probe, Z-gradient
Four channels (1H, 15N,
13C, 2H)
Auto Sampler: Sample Jet
Capacity: 5 racks of 96 samples (1 mm, 1.7 mm, 3
mm, 5 mm diameter) + 30 samples
Samples are kept at 4℃ in the
Sample Jet carousel
Controlled through IconNMR
Optimized for NMR screening applications such as
small-molecule screening, fragment-based
screening, DNA-encoded libraries
Gilson Sample Handler
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Gilson Sample Handler
Used for NMR High-Throughput Screening
1.7 mm NMR tubes; vol = 35 µL
3 mm NMR tubes; vol = 185 µL
5 mm NMR tubes; vol = 500 µL
Our Services & Equipment
Services
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Services
Free project consultation
Help with experimental design
Full support service
NMR Training
Our services include:
Collecting data
Data analysis
Data Interpretation
Access to internal and external users
The Core Facility runs 24/7
NMR Spectrometers
+
NMR Spectrometers
Three Bruker NMR spectrometers:
Bruker NEO 800MHz
Bruker Avance HD 700MHz
Bruker Avance III 600MHz
Our NMR Core facility is equipped with three NMR
spectrometers. For more information regarding the
specificity of each NMR spectrometer, please see section
NMR spectrometers below.
Bruker 800 MHz NMR Spectrometer
+
Bruker 800 MHz NMR Spectrometer
Magnet: Bruker 800 MHz US2 (Ultra Stabilized, Ultra
Shielded)
Console: NEO
Probes:
5 mm TXO CryoProbe Z-Gradient; 13C optimized (4171:1 ratio)
5 mm TCI CryoProbes Z-Gradient
5 mm TXI room temperature probe, Z-Gradient
Four channels (1H, 15N,
13C, 2H)
Optimized for protein structure, protein-protein
interaction, protein dynamic, protein-membrane
interaction, unstructured regions/proteins (IDPs).
Bruker 700 MHz NMR Spectrometer
+
Bruker 700 MHz NMR Spectrometer
Magnet: Bruker 700 MHz Ascend
Console: Avance III HD
Probes: 5 mm TCI CryoProbe, XYZ-Gradients
Four channels (1H, 15N,
13C, 2H)
Sample changer:
Cooled Sample Case: 24 samples (5mm/3mm tubes)
Samples are kept at 4℃ in the
sample case
Controlled through IconNMR
Optimized for routine protein studies
Bruker 600 MHz NMR Spectrometer
+
Bruker 600 MHz NMR Spectrometer
Magnet: Bruker 600 MHz US Plus
Console: NEO
Probes:
1.7 mm TCI Micro-CryoProbe, Z-gradient
5 mm TCI CryoProbe, Z-gradient
5 mm TXI room temperature probe, Z-gradient
Four channels (1H, 15N,
13C, 2H)
Auto Sampler: Sample Jet
Capacity: 5 racks of 96 samples (1 mm, 1.7 mm, 3
mm, 5 mm diameter) + 30 samples
Samples are kept at 4℃ in the
Sample Jet carousel
Controlled through IconNMR
Optimized for NMR screening applications such as
small-molecule screening, fragment-based
screening, DNA-encoded libraries
Gilson Sample Handler
+
Gilson Sample Handler
Used for NMR High-Throughput Screening
1.7 mm NMR tubes; vol = 35 µL
3 mm NMR tubes; vol = 185 µL
5 mm NMR tubes; vol = 500 µL
Services
We offer free project consultation to discuss project feasibility
(including sample preparation and NMR experiments) and offer
expert advice.
We offer a full service for researchers who may not be able to
perform their own experiments.
We train graduates, PhD students and post-doctoral fellows to run
their own NMR experiments and we provide support for the data
analysis when needed.
We collect, analyze data and send a full report when requested by
the researcher.
The NMR spectrometers are available to internal and external
researchers. The use of the NMR instruments independently is
contingent upon training and approval of each individual by the
NMR Core Facility Manager Dr. G. Seabrook.
FAQ
Please get in touch with Dr. Geneviève Seabrook for
information on user fees. Email address:
gseabroo@uhnresearch.ca
It will depend on the project. We will discuss the
choice of the spectrometer once we understand the goal
of the project.
We can screen a few thousands (~3000) of compounds in
just a few days.
